IRS-2 Antibody

Catalog Number:

48310

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#:Q9Y4H2

Form of Antibody:

1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

Storage/Stability:

Immunogen:

Amino acids 926-1130 of IRS-2 of human origin.

Purification:

Immunogen affinity purified

Specificity/Sensitivity:

Applications:

WB, IP, IF

Background:

IRS-2, originally described as 4PS, acts as a signaling intermediate downstream of the Insulin, IGF-1, IL-4, IL-9 and IL-13 receptors. In IRS-2-deficient mice, reduction in total PI 3-kinase activity by 30% and abolition of downstream activation of protein kinase C (PKC) ζ leads to the development of type 2 diabetes. Additionally, reconstitution with retroviral IRS-2 restores IRS-2/PI 3-kinase/PKC ζ signalling as well as glucose uptake. IRS-2 translocates to the nuclei of mouse embryo fibroblasts expressing the Insulin-like growth factor 1 receptor. Various mutations in the IGF-IR can result in an abrogation of or decrease in the translocation of IRS proteins to the nucleoli. IRS-2 is responsible for mitogen-activated protein kinase (MAPK) and protein kinase B (PKB) activation by Insulin and is the major adapter molecule linking the Insulin receptor to this step.

References:

appl_detail:

WB: 1:100-1:1,000


IP: 1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)

Datasheet PDF