NF-κB p105/p50 Antibody

Catalog Number:

48268

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#:P19838

Form of Antibody:

1*TBS (pH7.4), 0.5%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

Storage/Stability:

Immunogen:

A synthetic peptide corresponding to residues in the nuclear factor NF-κB p50 subunit.

Purification:

Protein A purified

Specificity/Sensitivity:

Applications:

WB, ICC, IHC, FC, IP

Background:

Nuclear factor κB (NF-κB) encompasses an important family of inducible transcriptional activators that regulate a wide variety of cellular and viral genes. The family members include p50, p52, p65 (RelA), c-Rel, and RelB. Association with inhibitory proteins of the IκB family retains NF-κB in the cytoplasm. Degradation of IκB proteins exposes the nuclear localization sequence (NLS), leading to nuclear translocation and subsequent binding of NF-B to DNA. The p105 precursor protein acts as an NFκB inhibitor, retaining Rel subunits in the cytoplasm of unstimulated cells. Upon stimulation by TNFα and IL -1α, degradation of p105 occurs; the Rel subunit is released and translocats to the nucleus. Additionally, p105 will be proteolytically processed by the 26S proteasome to produce a smaller, transcriptionally active form (p50).

References:

appl_detail:

WB: 1:1,000
IHC: 1:250-1:500
ICC: 1:250-1:500
IP: 1:30

Datasheet PDF