NF-κB p105/p50 Antibody

Catalog Number:

48171

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#:P19838

Form of Antibody:

1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

Storage/Stability:

Immunogen:

This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues in the nuclear factor NF-κB p50 subunit.

Purification:

Immunogen affinity purified

Specificity/Sensitivity:

Applications:

WB, ICC, IHC

Background:

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators.

References:

appl_detail:

WB: 1:500-1:1,000
IHC: 1:200
ICC: 1:200-1:500

Datasheet PDF