CD73 Antibody

Catalog Number:

48131

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#:P21589

Form of Antibody:

1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

Storage/Stability:

Immunogen:

This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region of human CD73 (aa 240-270).

Purification:

Peptide affinity purified.

Specificity/Sensitivity:

Applications:

WB, ICC, FC

Background:

CD73 (also designated ecto-5'-nucleotidase, E5NT, NT, NT5, NTE, eN and eNT) is a glycosyl-phosphatidylinositol (GPI)-anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 is a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins, CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants, yet elicits diverse biological function through differential regulation in endothelial cells (EC), subpopulations of B and T cells, germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts. For example, IgG mediated neutralization of CD73 interferes with lymphocyte adhesion to EC, and blocks aggregation of germinal center B cells and follicular dendritic cells. Furthermore, IgG-mediated targeting of lymphocyte CD73, but not of endothelial cell CD73, causes shedding of CD73 and tyrosine phosphorylation of proteins.

References:

appl_detail:

WB: 1:1,000-1:2,000

ICC: 1:50-1:200

FC: 1:50-1:100

Datasheet PDF