PKC alpha (Phospho-T638) Antibody

Catalog Number:

13428

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#:P17252

Form of Antibody:

1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

Storage/Stability:

Immunogen:

Synthetic phospho-peptide corresponding to residues surrounding Thr638 of human PKC alpha.

Purification:

ProA affinity purified

Specificity/Sensitivity:

Applications:

WB, ICC/IF, IHC, IP

Background:

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor-promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms (α, βI, βII, γ, δ, ε, ζ, η, θ, λ/ι, μ and ν). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC δ and ε are independent of Ca²⁺. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities.

References:

appl_detail:

WB: 1:1,000-5,000
IHC: 1:50-1:500
ICC: 1:50-1:500

Datasheet PDF