ptxA Polyclonal Antibody

Catalog Number:

42634

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P04977

Form of Antibody:

Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Storage/Stability:

Immunogen:

Recombinant Bordetella pertussis Pertussis toxin subunit 1 protein

Purification:

Caprylic Acid Ammonium Sulfate Precipitation purified

Specificity/Sensitivity:

The antibody detects endogenous level of total ptxA polyclonal antibody.

Applications:

WB

Background:

S1 is an NAD-dependent ADP-ribosyltransferase, which plays a crucial role in the pathogenesis of B.pertussis causing disruption of normal host cellular regulation. It catalyzes the ADP-ribosylation of a cysteine in the alpha subunit of host heterotrimeric G proteins. In the absence of G proteins it also catalyzes the cleavage of NAD+ into ADP-ribose and nicotinamide. It irreversibly uncouples the G-alpha GTP-binding proteins from their membrane receptors.

References:

[1] Cloning and sequencing of the pertussis toxin genes: operon structure and gene duplication.Nicosia A., Perugini M., Franzini C., Casagli M.C., Borri M.G., Antoni G., Almoni M., Neri P., Ratti G., Rappuoli R.Proc. Natl. Acad. Sci. U.S.A. 83:4631-4635(1

appl_detail:

Western blotting: 1:500 - 1:1000

Datasheet PDF