GLS2 Antibody

Catalog Number:

25272

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot:Q9UI32
Gene ID:27165

Form of Antibody:

Supplied in PBS containing 0.02% sodium azide.

Storage/Stability:

Immunogen:

Raised against a 18 amino acid peptide near the center terminus of human GLS2.

Purification:

Affinity chromatography purified via peptide column

Specificity/Sensitivity:

GLS2 antibody is predicted to not cross-react with other catenin family members. Multiple isoforms of GLS2 are known to exist.

Applications:

ELISA WB

Background:

Phosphate-activated glutaminase, also known as Glutaminase 2 (GLS2), was initially isolated from rat liver, although it has been shown to be expressed in other tissues. Like the functionally similar, larger kidney glutaminase, GLS2 catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Expression of GLS2 is increased by p53 under both stressed and nonstressed conditions, resulting in increased levels of glutamate and alpha-ketoglutarate, which in turn results in enhanced mitochondrial respiration and ATP generation. GLS2 also regulates antioxidant defense function in cells by increasing reduced glutathione levels and decreasing ROS-levels, suggesting that GLS2 acts as a mediator of p53’s role in antioxidant defense in addition to its role in energy metabolism.

References:

appl_detail:

Datasheet PDF