PLIN2（Phospho-Ser159 ) Antibody

Catalog Number:

58042

Amount:

100μg/100μl

Price:

$390

Swiss-Prot No:

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

synthetic phosphopeptide corresponding to residues surrounding Ser159 of human PLIN2

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phospholation site.

Specificity/Sensitivity:

PLIN2 （Phospho-Ser159 )antibody detects endogenous levels of PLIN2 only when phospholated at serine159 .

Applications:

Background:

Lipid droplets are surrounded by a single layer of polar, amphipathic phospholipids with structural proteins of the perilipin (PLIN) family, with PLIN1 being primarily an adipocyte protein and PLIN2 and PLIN3 being expressed ubiquitously [1] . Cells use these stored lipids as needed for a variety of functions, including energy production via fatty acid oxidation (also known as b-oxidation), membrane biogenesis for cell growth, protein modification, signaling, and secretion with lipoproteins. Glucose deprivation results in the binding of CHKα2 to PLIN2/3 and subsequent CHKa2-mediated PLIN2 Y232 phosphorylation. The protein kinase activity of CHKa2-depdendent PLIN2/3 phosphorylation is required for tumor cell proliferation and tumor growth [2].

References:

[1] Walther TC, Farese RV Jr. Lipid droplets and cellular lipid metabolism. Annu Rev Biochem. 2012; 81:687-714. doi: 10.1146/annurev-biochem-061009-102430. [2] Liu R, Lee JH, Li J, Yu R, Tan L, Xia Y, Zheng Y, Bian XL, Lorenzi PL, Chen Q, Lu Z. Choline kinase alpha 2 acts as a protein kinase to promote lipolysis of lipid droplets. Mol Cell. 2021 Jul 1;81(13):2722-2735.e9. doi: 10.1016/j.molcel.2021.05.005. [3]YingMeng,DongGuo,LimingLin,HongZhao,WeitingXu,ShudiLuo,XiaomingJiang,ShanLi,XuxiaoHe,RongxuanZhu,RongkaiShi,LiweiXiao,QingangWu,HaiyanHe,JingjingTao,HongfeiJiang,ZhengWang,PengboYao,DaqianXu&ZhimiLu.Glycolytic enzyme PFKL governs lipolysis by promoting lipid droplet-mitochondria tethering to enhance β-oxidation and tumor cell proliferation.Nature metabolism.https://doi.org/10.1038/s42255-024-01047-2. PMID:38773347

appl_detail:

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