PGC-1a（Methyl-Lys233 )Antibody

Catalog Number:

58035

Amount:

100μg/100μ

Price:

$390

Swiss-Prot No:

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

synthetic methylpeptide corresponding to residues surrounding Lys233 of human PGC-1a

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phospholation site.

Specificity/Sensitivity:

PGC-1a（Methyl-Lys233)antibody detects endogenous levels of PGC-1a only when methylated at lysine233 .

Applications:

Background:

Hypoxia, which occurs during tumor growth, triggers complex adaptive responses in which peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1a) plays a critical role in mitochondrial biogenesis and oxidative metabolism. KDM3A functions as an oxygen sensor and demethylates K224-monomethylated PGC-1a under normoxic conditions. Hypoxia inhibits KDM3A activity and increases PGC-1a K224 monomethylation, resulting in the inhibition of PGC-1a and PGC1a-dependent mitochondrial biogenesis, a decrease of ROS levels and apoptosis, and the promotion of brain tumor development [1]

References:

[1] Qian X, Li X, Shi Z, Bai X, Xia Y, Zheng Y, Xu D, Chen F, You Y, Fang J, Hu Z, Zhou Q, Lu Z. KDM3A Senses Oxygen Availability to Regulate PGC-1α-Mediated Mitochondrial Biogenesis. Mol Cell. 2019 Dec 19;76(6):885-895.e7. doi: 10.1016/j.molcel.2019.09.019.

appl_detail:

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