PRPS1/2（Acetyl-Lys29 )Antibody

Catalog Number:

58033

Amount:

100μg/100μ

Price:

$390

Swiss-Prot No:

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

synthetic acetylpeptide corresponding to residues surrounding Lys29 of human PRPS1/2

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phospholation site.

Specificity/Sensitivity:

PRPS1/2（Acetyl-Lys29 )antibody detects endogenous levels of PRPS1/2 only when acetylated at lysine29 .

Applications:

Background:

PRPS1 catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) that is essential for nucleotide synthesis. In the cytosol, CLOCK acetylates PRPS1/2 K29 and blocks HSC70-mediated and lysosome-dependent PRPS1/2 degradation. Stabilized PRPS1/2 promote de novo nucleotide synthesis and HCC cell proliferation and liver tumour growth. PRPS1/2 K29 acetylation are positively correlated with HCC poor prognosis [1]

References:

[1] Liu T, Wang Z, Ye L, Duan Y, Jiang H, He H, Xiao L, Wu Q, Xia Y, Yang M, Wu K, Yan M, Ji G, Shen Y, Wang L, Li L, Zheng P, Dong B, Shao F, Qian X, Yu R, Zhang Z, Lu Z, Xu D. Nucleus-exported CLOCK acetylates PRPS to promote de novo nucleotide synthesis and liver tumour growth. Nat Cell Biol. 2023 Feb;25(2):273-284. doi: 10.1038/s41556-022-01061-0.

appl_detail:

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