Bub3（Phospho-Tyr207 )Antibody

Catalog Number:

58030

Amount:

100μg/100μ

Price:

$390

Swiss-Prot No:

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

synthetic phosphopeptide corresponding to residues surrounding Tyr207 of human Bub3

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phospholation site.

Specificity/Sensitivity:

Bub3（Phospho-Tyr207)antibody detects endogenous levels of Bub3 only when phospholated at Tyrosine207 .

Applications:

Background:

PKM2 regulates G1-S phase transition by controlling cyclin D1 expression. PKM2 binds to the spindle checkpoint protein Bub3 during mitosis and phosphorylates Bub3 at Y207. This phosphorylation is required for Bub3-Bub1 complex recruitment to kinetochores, where it interacts with Blinkin and is essential for correct kinetochore-microtubule attachment, mitotic/spindle-assembly checkpoint, accurate chromosome segregation, cell survival and proliferation, and active EGF receptor-induced brain tumorigenesis. In addition, the level of Bub3 Y207 phosphorylation correlated with histone H3-S10 phosphorylation in human glioblastoma specimens and with glioblastoma prognosis [1] .

References:

[1] HeLa cells with PKM2 depletion and reconstituted expression of WT rPKM2 or rPKM2 P408/T409A were stained with the indicated antibodies. The cells in interphase and prometaphase were examined.

appl_detail:

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