PDHK1（Phospho-Thr388 )Antibody

Catalog Number:

58026

Amount:

100μg/100μ

Price:

$390

Swiss-Prot No:

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

synthetic phosphopeptide corresponding to residues surrounding Thr388 of human PDHK1

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phospholation site.

Specificity/Sensitivity:

PDHK1（Phospho-Thr388)antibody detects endogenous levels of PDHK1 only when phospholated at Threonine388 .

Applications:

Background:

Mitochondrial PGK1 acts as a protein kinase to phosphorylate pyruvate dehydrogenase kinase 1 (PDHK1) at T338, which activates PDHK1 to phosphorylate and inhibit the pyruvate dehydrogenase (PDH) complex. This reduces mitochondrial pyruvate utilization, suppresses reactive oxygen species production, increases lactate production, and promotes brain tumorigenesis. PDHK1 T338 phosphorylation levels correlate with poor prognosis in glioblastoma patients[1] .

References:

[1] Li X, Jiang Y, Meisenhelder J, Yang W, Hawke DH, Zheng Y, Xia Y, Aldape K, He J, Hunter T, Wang L, Lu Z. Mitochondria-Translocated PGK1 Functions as a Protein Kinase to Coordinate Glycolysis and the TCA Cycle in Tumorigenesis. Mol Cell. 2016 Mar 3;61(5):705-719. doi: 10.1016/j.molcel.2016.02.009. PMID: 26942675.

appl_detail:

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