PRPS1/2（Phospho-Ser180/183 )Antibody

Catalog Number:

58023

Amount:

100μg/100μ

Price:

$390

Swiss-Prot No:

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

synthetic phosphopeptide corresponding to residues surrounding Ser180/183 of human PRPS1/2

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phospholation site.

Specificity/Sensitivity:

PRPS1/2（Phospho- Ser180/183)antibody detects endogenous levels of PRPS1/2 only when phospholated at Serine180/183 .

Applications:

Background:

Glucose deprivation or hypoxia results in the AMPK-mediated phosphorylation of phosphoribosyl pyrophosphate synthetase 1 (PRPS1) S180 and PRPS2 S183, AMPK converts PRPS1/2 hexamers to monomers, and inhibits PRPS1/2 activity and subsequent nucleotide and NAD synthesis to maintain tumor cell growth and survival. The expression of nonphosphorylatable PRPS1/2 mutants greatly decreased cellular ATP and NADPH levels, increased reactive oxygen species (ROS) levels and cell apoptosis, and inhibited brain tumorigenesis [1] .

References:

[1] Qian X, Li X, Tan L, Lee JH, Xia Y, Cai Q, Zheng Y, Wang H, Lorenzi PL, Lu Z. Conversion of PRPS Hexamer to Monomer by AMPK-Mediated Phosphorylation Inhibits Nucleotide Synthesis in Response to Energy Stress. Cancer Discov. 2018 Jan;8(1):94-107. doi: 10.1158/2159-8290.CD-17-0712.

appl_detail:

Datasheet PDF