MLC2 （Phospho-Tyr118 )Antibody

Catalog Number:

58017

Amount:

100μg/100μ

Price:

$390

Swiss-Prot No:

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

synthetic phosphopeptide corresponding to residues surrounding Tyr118 of human MLC2

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phospholation site.

Specificity/Sensitivity:

MLC2 （Phospho-Tyr118 )antibody detects endogenous levels of MLC2 only when phospholated at Tyrosine118 .

Applications:

Background:

MLC2 protein can be phosphorylated by PKM2 at Y118, which primes the binding of ROCK2 to MLC2 subsequent ROCK2-dependent MLC2 S15 phosphorylation. PKM2-regulated MLC2 phosphorylation plays a pivotal role in cytokinesis, cell proliferation and brain tumour development [1] .

References:

[1] Jiang Y, Wang Y, Wang T, Hawke DH, Zheng Y, Li X, Zhou Q, Majumder S, Bi E, Liu DX, Huang S, Lu Z. PKM2 phosphorylates MLC2 and regulates cytokinesis of tumour cells. Nat Commun. 2014 Nov 21; 5:5566. doi: 10.1038/ncomms6566.

appl_detail:

Datasheet PDF