CLOCK（Phospho-Ser106 )Antibody

Catalog Number:

58016

Amount:

100μg/100μ

Price:

$390

Swiss-Prot No:

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

synthetic phosphopeptide corresponding to residues surrounding Ser106 of human CLOCK

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phospholation site.

Specificity/Sensitivity:

CLOCK（Phospho-Ser106 )antibody detects endogenous levels of CLOCK only when phospholated at Serine106 .

Applications:

Background:

The transcriptional activity of CLOCK governs circadian rhythms, and impairment of the circadian clock has been linked to cancer development. Activation of receptor tyrosine kinases promoted the binding of CK2α to CLOCK and the subsequent CK2α-mediated CLOCK S106 phosphorylation. This phosphorylation disassembled CLOCK–BMAL1 heterodimerization, leading to suppression of the CLOCK/BMAL1-mediated expression of the downstream genes in HCC cells[[1] Liu T, Wang Z, Ye L, Duan Y, Jiang H, He H, Xiao L, Wu Q, Xia Y, Yang M, Wu K, Yan M, Ji G, Shen Y, Wang L, Li L, Zheng P, Dong B, Shao F, Qian X, Yu R, Zhang Z, Lu Z, Xu D. Nucleus-exported CLOCK acetylates PRPS to promote de novo nucleotide synthesis and liver tumour growth. Nat Cell Biol. 2023 Feb;25(2):273-284. doi: 10.1038/s41556-022-01061-0. ][1].

References:

[1] Liu T, Wang Z, Ye L, Duan Y, Jiang H, He H, Xiao L, Wu Q, Xia Y, Yang M, Wu K, Yan M, Ji G, Shen Y, Wang L, Li L, Zheng P, Dong B, Shao F, Qian X, Yu R, Zhang Z, Lu Z, Xu D. Nucleus-exported CLOCK acetylates PRPS to promote de novo nucleotide synthesis and liver tumour growth. Nat Cell Biol. 2023 Feb;25(2):273-284. doi: 10.1038/s41556-022-01061-0.

appl_detail:

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