CHKα2 （Acetyl-Lys247 )Antibody

Catalog Number:

58011

Amount:

100μg/100μ

Price:

$390

Swiss-Prot No:

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

synthetic acetylpeptide corresponding to residues surrounding Lys247 of human CHKα2

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylpeptide. The antibody against non-acetylpeptide was removed by chromatography using non-acetylpeptide corresponding to the acetylation site.

Specificity/Sensitivity:

CHKα2 （Acetyl-Lys247 )antibody detects endogenous levels of CHKα2 only when acetylated at lysine247 .

Applications:

Background:

Glucose deprivation induced AMPK-dependent CHKα2 phosphorylation could induce recruitment of KAT5 for acetylation of CHKα2 at K247. Acetylation of CHKα2 disrupted the CHKα2 dimer and exposed the hydrophobic I186/L187 residues of the helix in the interacting interface of the dimer to bind to lipid droplet protein PLIN2/3. Levels of CHKα2 K247 acetylation are positively correlated with one another in human glioblastoma specimens and are associated with poor prognosis in glioblastoma patients [1]

References:

[1] Liu R, Lee JH, Li J, Yu R, Tan L, Xia Y, Zheng Y, Bian XL, Lorenzi PL, Chen Q, Lu Z. Choline kinase alpha 2 acts as a protein kinase to promote lipolysis of lipid droplets. Mol Cell. 2021 Jul 1;81(13):2722-2735.e9. doi: 10.1016/j.molcel.2021.05.005.

appl_detail:

Datasheet PDF