CHKα2 （Phospho-Ser279 )Antibody

Catalog Number:

58010

Amount:

100μg/100μl

Price:

$390

Swiss-Prot No:

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

synthetic phosphopeptide corresponding to residues surrounding Ser279 of human CHKα2

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificity/Sensitivity:

CHKα2 （Phospho-Ser279 )antibody detects endogenous levels of CHKα2 only when phosphorylated at serine279 .

Applications:

Background:

CHKa promotes tumor cell proliferation and survival. Its overexpression has been detected in 40%–60% of human tumors and is correlated with prognosis in early-stage non-small cell lung cancer, hepatocellular carcinoma (HCC), and prostate cancers [1] Glucose deprivation could induce CHKa2 S279 phosphorylation in Huh7 cell. Levels of CHKα2 S279 phosphorylation and PLIN2/3 phosphorylation are positively correlated with one another in human glioblastoma specimens and are associated with poor prognosis in glioblastoma patients [2] .

References:

[1] Challapalli A, Trousil S, Hazell S, Kozlowski K, Gudi M, Aboagye EO, Mangar S. Exploiting altered patterns of choline kinase-alpha expression on human prostate tissue to prognosticate prostate cancer. J Clin Pathol. 2015 Sep;68(9):703-9. doi: 10.1136/jclinpath-2015-202859. [2] Liu R, Lee JH, Li J, Yu R, Tan L, Xia Y, Zheng Y, Bian XL, Lorenzi PL, Chen Q, Lu Z. Choline kinase alpha 2 acts as a protein kinase to promote lipolysis of lipid droplets. Mol Cell. 2021 Jul 1;81(13):2722-2735.e9. doi: 10.1016/j.molcel.2021.05.005.

appl_detail:

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