PFKFB3 Antibody

Catalog Number:

49656

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#:Q16875

Form of Antibody:

1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

Storage/Stability:

Immunogen:

Recombinant protein

Purification:

ProA affinity purified

Specificity/Sensitivity:

Applications:

WB, ICC, IHC, FC, IP

Background:

Among the enzymes playing role in glycolysis,four allosteric PFKFB enzymes 1C4 expressed by four independent PFKFB genes, catalyze the rate-limiting phosphorylation of fructose-6-phosphate to fructose-1, 6-bisphosphate, using ATP as the energy source in the glycolysis pathway. Among these four allosteric enzymes, PFKFB3 enzyme retains the highest Kinase/Biphosphatase activity ratio and is expressed by PFKFB3 gene which has been demonstrated to be highly expressed in leukemic cells and in solid tumors. Moreover, mitogenic, hypoxic and inflammatory conditions have an inductive effect on the expression of PFKFB3. Hence upregulation of PFKFB genes specific to cancer cells compared to their normal counterparts (from the same patients) with more robust over-expression in breast and lung cancer make it a more appropriate target.

References:

appl_detail:

WB: 1:500-1:1,000
IHC: 1:50-1200
ICC: 1:50-1200

FC: 1:50-1:100

Datasheet PDF