JNK Antibody

Catalog Number:

48335

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#:P45983

Form of Antibody:

1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

Storage/Stability:

Immunogen:

Amino acids 1-384 representing full length JNK1 of human origin.

Purification:

Immunogen affinity purified

Specificity/Sensitivity:

Applications:

WB, IP, IF, FCM

Background:

c-Jun N-terminal kinases (JNKs) phosphorylate and augment transcriptional activity of c-Jun. JNKs originate from three genes that yield ten isoforms through alternative mRNA splicing, including JNK1α1, JNK1β1, JNK2α1, JNK2β1 and JNK3α1, which represent the p46 isoforms, and JNK1α2, JNK1β2, JNK2α2, JNK2β2 and JNK3β2, which represent the p54 isoforms. JNKs coordinate cell responses to stress and influence regulation of cell growth and transformation. The human JNK1 (PRKM8, SAPK1, MAPK8) gene maps to chromosome 10q11.22 and shares 83% amino acid identity with JNK2. JNK1 is necessary for normal activation and differentiation of CD4 helper T (TH) cells into TH1 and TH2 effector cells. Capsaicin activates JNK1 and p38 in Ras-transformed human breast epithelial cells. Nitrogen oxides (NOx) upregulate JNK1 in addition to c-Fos, c-Jun and other signaling kinases, including MEKK1 and p38.

References:

appl_detail:

WB: 1:100-1:1,000


IP: 1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)
FC: 1 μg per 1 x 106 cells

Datasheet PDF