iNOS Antibody

Catalog Number:

48309

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#:P35228

Form of Antibody:

1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

Storage/Stability:

Immunogen:

peptide

Purification:

Immunogen affinity purified

Specificity/Sensitivity:

Applications:

WB, IP, IF, IHC(P)

Background:

Nitric oxide (NO) has a broad range of biological activities and has been implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOSs), the enzymes responsible for synthesis of NO, contain an N-terminal oxygenase domain and a C-terminal reductase domain. NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells. The inducible form of NOS, NOS2 (also designated iNOS), is Ca2+-independent and is expressed in a broad range of cell types.

References:

appl_detail:

WB: 1:100-1:1,000
IHC: 1:50-1:500

IP: 1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)

Datasheet PDF