FOXO3a(Phospho-S253) Antibody

Catalog Number:

13372

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#:O43524

Form of Antibody:

1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

Storage/Stability:

Immunogen:

Synthetic phospho-peptide corresponding to residues surrounding Ser253 of human FOXO3a.

Purification:

ProA affinity purified

Specificity/Sensitivity:

Applications:

WB, IHC

Background:

FKHRL1 (for forkhead in rhabdomyosarcoma) is a member of the FKHR subfamily of forkhead transcription factors. Transcriptional activation of FKHR proteins is regulated by the serine/threonine kinase Akt1, which phosphorylates FKHRL1 at threonine-32 and serine-253. Phosphorylation by Akt1 negatively regulates FKHRL1 by promoting its export from the nucleus. Phosphorylated FKHRL1 associates with 14-3-3 proteins and this complex is retained in the cytoplasm. Growth factor withdrawal stimulates FKHRL1 dephosphorylation and nuclear translocation, leading to FKHR-induced gene-specific transcriptional activation. Within the nucleus, dephospho-rylated FKHRL1 triggers apoptosis by inducing the expression of genes that are critical for cell death.

References:

appl_detail:

WB: 1:1,000-5,000
IHC: 1:50-1:200

Datasheet PDF