Smad3(Phospho-S423/S425) Antibody

Catalog Number:

13370

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#:P84022

Form of Antibody:

1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

Storage/Stability:

Immunogen:

Synthetic phospho-peptide corresponding to residues surrounding Ser423 and 425 of human Smad3.

Purification:

ProA affinity purified

Specificity/Sensitivity:

Applications:

WB, ICC/IF, IHC

Background:

Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members. Human Smad3 is subject to phosphorylation by TGFβ on specific amino acid residues, including Ser 208.

References:

appl_detail:

WB: 1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200

Datasheet PDF