Creb (Phospho-Ser 133) Antibody

Catalog Number:

13323

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#:P16220

Form of Antibody:

1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

Storage/Stability:

Immunogen:

A short amino acid sequence containing Ser 133 phosphorylated CREB-1 of human origin.

Purification:

Immunogen affinity purified

Specificity/Sensitivity:

Applications:

WB, IP, IF, IHC(P)

Background:

Eukaryotic gene transcription is regulated by sequence-specific transcription factors that bind modular cis acting promoter and enhancer elements. The ATF/CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. Although CREB can bind to DNA in an unphosphorylated state, it cannot activate transcription. Phosphorylation of CREB on Ser133 by protein kinase A facilitates its interaction with the CREB-binding protein (CBP) and activates the basal transcription complex. CREB functions in neoglucogenesis through interactions with the nuclear coactivator PGC-1. CREB may play a role in the pathogenesis of type II diabetes and dilated cardiomyopathy. The gene encoding CREB-1 maps to human chromosome 2q32.3-q34.

References:

appl_detail:

WB: 1:100-1:1,000
IHC: 1:50-1:500

IP: 1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)

Datasheet PDF