GTF2A2 Antibody

Catalog Number:

47329

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#:P52657
NCBI Gene ID:2958
Gene Accssion:BC000287

Form of Antibody:

Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

Storage/Stability:

Immunogen:

Fusion protein of human GTF2A2

Purification:

Antigen affinity purification

Specificity/Sensitivity:

The antibody detects endogenous levels of total GTF2A2 protein.

Applications:

WB, IHC

Background:

Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and the general initiation factors TFIIA, TFIIB (MIM 189963), TFIID (MIM 313650), TFIIE (MIM 189962), TFIIF (MIM 189968), TFIIG/TFIIJ, and TFIIH (MIM 189972). The first step involves recognition of the TATA element by the TATA-binding subunit (TBP; MIM 600075) and may be regulated by TFIIA, a factor that interacts with both TBP and a TBP-associated factor (TAF; MIM 600475) in TFIID. TFIIA has 2 subunits (43 and 12 kD) in yeast and 3 subunits in higher eukaryotes. In HeLa extracts, it consists of a 35-kD alpha subunit and a 19-kD beta subunit encoded by the N- and C-terminal regions of GTF2A1 (MIM 600520), respectively, and a 12-kD gamma subunit encoded by GTF2A2 (DeJong et al., 1995 [PubMed 7724559]).

References:

appl_detail:

WB dilution:1:500-2000
IHC dilution:1: 25-100

Datasheet PDF