MAPKAP1 Polyclonal Antibody

Catalog Number:

42671

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q9BPZ7

Form of Antibody:

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage/Stability:

Immunogen:

Recombinant human Target of rapamycin complex 2 subunit MAPKAP1 protein (1-290aa)

Purification:

Antigen Affinity Purified

Specificity/Sensitivity:

The antibody detects endogenous level of total MAPKAP1 polyclonal antibody.

Applications:

WB

Background:

Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex.

References:

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."The MGC Project Team Genome Res. 14:2121-2127(2004). [2]"SIN1/MIP1 maintains rictor-mTOR complex integrity and regulates Akt phosphorylation a

appl_detail:

Western blotting: 1:500 - 1:2000

Datasheet PDF