E3 ubiquitin-protein ligase MIB1 Polyclonal Antibody

Catalog Number:

42567

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q86YT6

Form of Antibody:

Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Storage/Stability:

Immunogen:

Recombinant human E3 ubiquitin-protein ligase MIB1 protein

Purification:

Caprylic Acid Ammonium Sulfate Precipitation purified

Specificity/Sensitivity:

The antibody detects endogenous level of total E3 ubiquitin-protein ligase MIB1 polyclonal antibody.

Applications:

WB

Background:

E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis (By similarity). Involved in ubiquitination of centriolar satellite CEP131, CEP290 and PCM1 proteins and hence inhibits primary cilium formation in proliferating cells. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation.

References:

[1] "Mind bomb is a ubiquitin ligase that is essential for efficient activation of Notch signaling by Delta."Itoh M., Kim C.-H., Palardy G., Oda T., Jiang Y.-J., Maust D., Yeo S.-Y., Lorick K., Wright G.J., Ariza-McNaughton L., Weissman A.M., Lewis J., Ch

appl_detail:

Western blotting: 1:500 - 1:1000

Datasheet PDF