L1 Polyclonal Antibody

Catalog Number:

42503

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P06794

Form of Antibody:

Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Storage/Stability:

Immunogen:

Recombinant HPV18 Major capsid protein L1 protein

Purification:

Caprylic Acid Ammonium Sulfate Precipitation purified

Specificity/Sensitivity:

The antibody detects endogenous level of total L1 polyclonal antibody.

Applications:

WB IHC

Background:

Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on the basement membrane to provide initial virion attachment to target cells. Basement membrane is exposed only after epithelium trauma. Additionally, the alpha6 integrin complexed with either beta1 or beta4 integrin has been proposed to act as a coreceptor recognized by L1. Once attached, integrin complexed with beta4 integrin has been proposed to act as a coreceptor recognized by L1. Once attached, the virion enters the host cell via clathrin-mediated endocytosis and the genomic DNA is released to the host nucleus. The virion assembly takes place within the cell nucleus. Encapsulates the genomic DNA together with protein L2.

References:

[1] "Human papillomavirus type 16 DNA sequence."Seedorf K., Krammer G., Durst M., Suhai S., Rowekamp W.G.Virology 145:181-185(1985) [2] "Nucleotide sequences and further characterization of human papillomavirus DNA present in the CaSki, Si

appl_detail:

Western blotting: 1:500 - 1:1000

Datasheet PDF