NGAL Polyclonal Antibody

Catalog Number:

42467

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P80188

Form of Antibody:

Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Storage/Stability:

Immunogen:

Recombinant human NGAL protein

Purification:

Caprylic Acid Ammonium Sulfate Precipitation purified

Specificity/Sensitivity:

The antibody detects endogenous level of total NGAL polyclonal antibody.

Applications:

WB

Background:

Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.

References:

[1] "Molecular cloning and expression of a cDNA encoding NGAL: a lipocalin expressed in human neutrophils."Bundgaard J.R., Sengelov H., Borregaard N., Kjeldsen L.Biochem. Biophys. Res. Commun. 202:1468-1475(1994) [2] "Molecular characterization and patt

appl_detail:

Western blotting: 1:500 - 1:1000

Datasheet PDF