Rho guanine nucleotide exchange factor 18 Polyclonal Antibody

Catalog Number:

42430

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q6ZSZ5

Form of Antibody:

Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Storage/Stability:

Immunogen:

Recombinant human Rho guanine nucleotide exchange factor 18 protein

Purification:

Caprylic Acid Ammonium Sulfate Precipitation purified

Specificity/Sensitivity:

The antibody detects endogenous level of total Rho guanine nucleotide exchange factor 18 polyclonal antibody.

Applications:

WB

Background:

Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription, cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). p114RgoGEF cts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. It may play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It also act as a GEF for RAC1, inducing production of reactive oxygen species (ROS), but does not act as a GEF for CDC42. The G protein beta-gamma (Gbetagamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change and ROS production.

References:

[1] "Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."Nagase T., Ishikawa K., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.DNA

appl_detail:

Western blotting: 1:500 - 1:1000

Datasheet PDF