Ubiquitin thioesterase OTUB1 Polyclonal Antibody

Catalog Number:

42398

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q96FW1

Form of Antibody:

Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Storage/Stability:

Immunogen:

Recombinant human Ubiquitin thioesterase OTUB1 protein

Purification:

Caprylic Acid Ammonium Sulfate Precipitation purified

Specificity/Sensitivity:

The antibody detects endogenous level of total Ubiquitin thioesterase OTUB1 polyclonal antibody.

Applications:

WB

Background:

Hydrolase that can specifically remove 'Lys-48'-linked conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 stabilizes RNF128 and promotes anergy.

References:

[1] "Otubains: a new family of cysteine proteases in the ubiquitin pathway."Balakirev M.Y., Tcherniuk S.O., Jaquinod M., Chroboczek J.EMBO Rep. 4:517-522(2003) [2] "Two isoforms of otubain 1 regulate T cell anergy via GRAIL."Soares L., Seroogy C., Skren

appl_detail:

Western blotting: 1:500 - 1:1000

Datasheet PDF