Pyruvate kinase PKM Polyclonal Antibody

Catalog Number:

42380

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P14618

Form of Antibody:

Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Storage/Stability:

Immunogen:

Recombinant human Pyruvate kinase PKM protein

Purification:

Caprylic Acid Ammonium Sulfate Precipitation purified

Specificity/Sensitivity:

The antibody detects endogenous level of total Pyruvate kinase PKM polyclonal antibody.

Applications:

IHC

Background:

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

References:

[1] "Human M2-type pyruvate kinase: cDNA cloning, chromosomal assignment and expression in hepatoma." Tani K., Yoshida M.C., Satoh H., Mitamura K., Noguchi T., Tanaka T., Fujii H., Miwa S.Gene 73:509-516(1988) [2] "Cytosolic thyroid h

appl_detail:

Immunohistochemistry: 1:20 - 1:200

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