RAD51D Polyclonal Antibody

Catalog Number:

42306

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: O75771

Form of Antibody:

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage/Stability:

Immunogen:

Recombinant human DNA repair protein RAD51 homolog 4 protein(1-328aa)

Purification:

Antigen Affinity Purified

Specificity/Sensitivity:

The antibody detects endogenous level of total RAD51D polyclonal antibody.

Applications:

WB IHC

Background:

Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. Bind to single-stranded DNA (ssDNA) and has DNA-dependent ATPase activity. Part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Involved in telomere maintenance. The BCDX2 subcomplex XRCC2:RAD51D can stimulate Holliday junction resolution by BLM.

References:

[1]Structural and functional characterization of the N-terminal domain of human Rad51D." Kim Y.M., Choi B.S.Int. J. Biochem. Cell Biol. 43:416-422(2011). [2]Rad51 paralog complexes BCDX2 and CX3 act at different stages in the BRCA1-BRCA2-dependent homolog

appl_detail:

Western blotting: 1:500 - 1:1000
Immunohistochemistry: 1:20 - 1:200

Datasheet PDF