Replication protein A 32 kDa subunit Polyclonal Antibody

Catalog Number:

42269

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P15927

Form of Antibody:

Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Storage/Stability:

Immunogen:

Recombinant human Replication protein A 32 kDa subunit protein

Purification:

Caprylic Acid Ammonium Sulfate Precipitation purified

Specificity/Sensitivity:

The antibody detects endogenous level of total Replication protein A 32 kDa subunit polyclonal antibody.

Applications:

WB IHC

Background:

Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

References:

[1] "The primary structure of the 32-kDa subunit of human replication protein A."Erdile L.F., Wold M.S., Kelly T.J.J. Biol. Chem. 265:3177-3182(1990) [2] "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L

appl_detail:

Western blotting: 1:500 - 1:1000
Immunohistochemistry: 1:20 - 1:200

Datasheet PDF