Cyclin-dependent kinase 4 Polyclonal Antibody

Catalog Number:

42545

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P11802

Form of Antibody:

Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Storage/Stability:

Immunogen:

Recombinant human Cyclin-dependent kinase 4

Purification:

Caprylic Acid Ammonium Sulfate Precipitation purified

Specificity/Sensitivity:

The antibody detects endogenous level of total Cyclin-dependent kinase 4 polyclonal antibody.

Applications:

IHC

Background:

Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

References:

[1] Hanks S.K.Submitted (FEB-1987) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [MRNA]. [2] "Transcript mapping in a 46-kb sequenced region at the core of 12q13.3 amplification in human cancers."Elkahloun A.G., Krizman D.B.

appl_detail:

Immunohistochemistry: 1:20 - 1:200

Datasheet PDF