Thiopurine S-methyltransferase Polyclonal Antibody

Catalog Number:

42134

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P51580

Form of Antibody:

Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Storage/Stability:

Immunogen:

Recombinant human Thiopurine S-methyltransferase protein

Purification:

Caprylic Acid Ammonium Sulfate Precipitation purified

Specificity/Sensitivity:

The antibody detects endogenous level of total Thiopurine S-methyltransferase polyclonal antibody.

Applications:

WB IHC

Background:

TPMT is an enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals. A pseudogene for this locus is located on chromosome 18q.

References:

[1] "Human thiopurine methyltransferase: molecular cloning and expression of T84 colon carcinoma cell cDNA."Honchel R., Aksoy I.A., Szumlanski C., Wood T.C., Otterness D.M., Wieben E.D., Weinshilboum R.M.Mol. Pharmacol. 43:878-887(1993) [2]

appl_detail:

Western blotting: 1:500 - 1:1000
Immunohistochemistry: 1:20 - 1:200

Datasheet PDF