ACTL6B Polyclonal Antibody

Catalog Number:

42057

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: O94805

Form of Antibody:

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage/Stability:

Immunogen:

Recombinant human Actin-like protein 6B protein(1-280aa)

Purification:

Antigen Affinity Purified

Specificity/Sensitivity:

The antibody detects endogenous level of total ACTL6B polyclonal antibody.

Applications:

WB IHC

Background:

Belongs to the chromatin remodeling brain-specific BAF (bBAF) complex, as such plays a role in remodeling mononucleosomes in an ATP-dependent fashion. Belongs to the neuron-specific chromatin remodeling complex (nBAF complex) and is required for postmitotic neural development and dendritic outgrowth. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. ACTL6B/BAF53B is not essential for assembly of the nBAF complex but is required for targeting the complex and CREST to the promoter of genes essential for dendritic growth .

References:

[1]The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project TeamGenome Res. 14:2121-2127(2004). [2]Complete sequencing and characterization of 21,243 full-length human cDNAs."Ota T., Suz

appl_detail:

Western blotting: 1:500 - 1:1000
Immunohistochemistry: 1:20 - 1:200

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