ATP synthase subunit delta Polyclonal Antibody

Catalog Number:

42141

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P25705

Form of Antibody:

Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Storage/Stability:

Immunogen:

Recombinant human ATP synthase subunit delta, mitochondrial protein

Purification:

Caprylic Acid Ammonium Sulfate Precipitation purified

Specificity/Sensitivity:

The antibody detects endogenous level of total ATP synthase subunit delta polyclonal antibody.

Applications:

WB IHC

Background:

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP turnover in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F1 domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

References:

[1]"Molecular cloning of an import precursor of the delta-subunit of the human mitochondrial ATP synthase complex."Jordan E.M., Breen G.A.M.Biochim. Biophys. Acta 1130:123-126(1992) [2]"Cloning of human full open reading frames in Gateway(TM) system entr

appl_detail:

Western blotting: 1:500 - 1:1000
Immunohistochemistry: 1:20 - 1:200

Datasheet PDF