26S proteasome non-ATPase regulatory subunit 11 Polyclonal Antibody

Catalog Number:

42132

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: O00231

Form of Antibody:

Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Storage/Stability:

Immunogen:

Recombinant human 26S proteasome non-ATPase regulatory subunit 11 protein

Purification:

Caprylic Acid Ammonium Sulfate Precipitation purified

Specificity/Sensitivity:

The antibody detects endogenous level of total 26S proteasome non-ATPase regulatory subunit 11 polyclonal antibody.

Applications:

WB IHC

Background:

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits.Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator.

References:

[1] "Molecular cloning and expression of subunit 9 of the 26S proteasome."Hoffman L., Rechsteiner M.FEBS Lett. 404:179-184(1997) [2] "cDNA cloning and functional analysis of p44.5 and p55, two regulatory subunits of the 26S proteasome."Saito A., Watanabe

appl_detail:

Western blotting: 1:500 - 1:1000
Immunohistochemistry: 1:20 - 1:200

Datasheet PDF