TICAM1 antibody

Catalog Number:

38206

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q8IUC6
NCBI Gene ID: 148022

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Recombinant protein of human TICAM1.

Purification:

Antibodies were purified by affinity purification using immunogen.

Specificity/Sensitivity:

The antibody detects endogenous level of total TICAM1 antibody.

Applications:

WB

Background:

Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-3). TLRs recognize conserved motifs found in various pathogens and mediate defense responses. Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes. The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the TIR domain. Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains including MyD88 (myeloid differentiation factor), MAL/TIRAP (MyD88-adaptor-like/TIR-associated protein), TRIF (Toll-receptor-associated activator of interferon), and TRAM (Toll-receptor-associated molecule). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK. Activation of IKK leads to the degradation of IκB that normally maintains NF-κB inactivity by sequestering it in the cytoplasm. TRIF (also termed TICAM-1) is a TIR-domain adaptor protein described to activate NF-κB, IRF3 and trigger IFN-β production (4,5). Studies using dominant negative forms of TRIF and siRNA targeting TRIF show that TRIF functions downstream of both TLR3 and TLR4 in response to dsRNA and LPS respectively (4-6). TRIF recruits TRAF6-TAK1-TAB2 to the receptor complex which leads to NF-κB activation (7). In addition, TRIF can trigger signaling of that lead to the induction of apoptosis (8).

References:

appl_detail:

WB 1:500 - 1:2000

Datasheet PDF