HMGCS1 Antibody

Catalog Number:

37622

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q01581
NCBI Gene ID: 3157
Gene Accssion: NP_001091742

Form of Antibody:

Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

Storage/Stability:

Immunogen:

Synthetic peptide corresponding to a region derived from internal residues of human 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble)

Purification:

Antigen affinity purification.

Specificity/Sensitivity:

The antibody detects endogenous levels of total HMGCS1 protein.

Applications:

WB IHC

Background:

HMG-CoA Synthase exists as both a mitochondrial (mHMGCS) and cytoplasmic (cHMGCS) enzyme that condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA. The HMG-CoA produced by cHMGCS is transformed into mevalonate by HMG-CoA reductase, which starts isoprenoid biosynthesis. End products of the isoprenoid pathway include cholesterol, ubiquinone, dolichol, isopentenyl adenosine and farnesyl groups. mHMGCS, together with HMG-CoA Lyase, is responsible for ketone body biosynthesis. mHMGCS is expressed in liver and kidney. Fasting, cAMP and fatty acids increase the level of transcription of mHMGCS, while feeding and insulin repress it. A regulatory element within the mHMGCS promoter confers transcriptional regulation by PPAR, RXR, COUP-TF and HNF-4.

References:

appl_detail:

Western blotting: 1:500-1:2000
Immunohistochemistry: 1:50-1:200

Datasheet PDF