ESPL1 Antibody

Catalog Number:

37557

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q14674
NCBI Gene ID: 9700
Gene Accssion: NP_036423

Form of Antibody:

Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

Storage/Stability:

Immunogen:

Synthetic peptide corresponding to a region derived from internal residues of human extra spindle pole bodies homolog 1 (S. cerevisiae)

Purification:

Antigen affinity purification.

Specificity/Sensitivity:

The antibody detects endogenous levels of total ESPL1 protein.

Applications:

WB IHC

Background:

Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 or STAG2 in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 by ESPL1, or separase, which initiates the final separation of sister chromatids.

References:

appl_detail:

Western blotting: 1:200-1:1000
Immunohistochemistry: 1:25-1:100

Datasheet PDF