ERN2 Antibody

Catalog Number:

37554

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q76MJ5
NCBI Gene ID: 10595
Gene Accssion: NP_150296

Form of Antibody:

Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

Storage/Stability:

Immunogen:

Synthetic peptide corresponding to a region derived from internal residues of human endoplasmic reticulum to nucleus signaling 2

Purification:

Antigen affinity purification.

Specificity/Sensitivity:

The antibody detects endogenous levels of total ERN2 protein.

Applications:

IHC

Background:

The accumulation of unfolded proteins within the endoplasmic recticulum (ER) of yeast and mammalian cells activates the unfolded protein response (UPR) pathway and leads to the transcription of ER-specific genes involved in protein folding. The activation of the UPR requires the ER transmembrane kinase IRE1p (for inositol-requiring and ER-to-nucleus signaling protein). IRE1α and IRE1β are two mammalian homologs of the yeast IRE1p. These related proteins localize to the ER lumen and contain both a short transmembrane domain that spans the ER membrane and a cytosolic Ser/Thr kinase domain. IRE1 activation involves the oligomerization and trans-phosphorylation of the cytosolic portion of the proteins, which then potentiates its intrinsic kinase activity and, in turn, stimulates transcription of UPR-targeted genes.

References:

appl_detail:

Immunohistochemistry: 1:50-1:200

Datasheet PDF