MCAM Antibody

Catalog Number:

37174

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P43121
NCBI Gene ID: 4162
Gene Accssion: NP_006491

Form of Antibody:

Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

Storage/Stability:

Immunogen:

Synthetic peptide corresponding to a region derived from internal residues of human melanoma cell adhesion molecule

Purification:

Antigen affinity purification.

Specificity/Sensitivity:

The antibody detects endogenous levels of total MCAM protein.

Applications:

WB IHC

Background:

CD146 (cluster of differentiation 146) also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. In humans, the CD146 protein is encoded by the MCAM gene. Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.

References:

appl_detail:

Western blotting: 1:500-1:2000
Immunohistochemistry: 1:50-1:200

Datasheet PDF