RBFOX3 Antibody

Catalog Number:

36998

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: A6NFN3
NCBI Gene ID: 146713
Gene Accssion: NP_001076044

Form of Antibody:

Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

Storage/Stability:

Immunogen:

Synthetic peptide corresponding to a region derived from internal residues of human RNA binding protein, fox-1 homolog (C. elegans) 3

Purification:

Antigen affinity purification.

Specificity/Sensitivity:

The antibody detects endogenous levels of total RBFOX3 protein.

Applications:

WB IHC

Background:

?Immunoprecipitation and mass spectrometry of the two major NeuN species at 45¨C50 kDa identified both as the RNA binding protein Rbfox3 (a member of the Fox family of alternative splicing factors), confirming and extending the identification of the 45 kDa band as Rbfox3. Mapping of the anti-NeuN reactive epitopes in both R3hdm2 and Rbfox3 reveals a common proline- and glutamine-rich domain that lies at the N-terminus of the Rbfox3 protein. Nuclear Rbfox3 isoforms can also enhance the inclusion of cryptic exons in the Rbfox2 mRNA, resulting in nonsense-mediated decay of the message, thereby contributing to the negative regulation of Rbfox2 by Rbfox3 through a novel mechanism.

References:

appl_detail:

Western blotting: 1:200-1:1000
Immunohistochemistry: 1:15-1:50

Datasheet PDF