MCM4 Antibody

Catalog Number:

36977

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P33991
NCBI Gene ID: 4173
Gene Accssion: NP_877423

Form of Antibody:

Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

Storage/Stability:

Immunogen:

Synthetic peptide corresponding to a region derived from internal residues of human Minichromosome maintenance complex component 4

Purification:

Antigen affinity purification.

Specificity/Sensitivity:

The antibody detects endogenous levels of total MCM4 protein.

Applications:

WB IHC

Background:

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.

References:

appl_detail:

Western blotting: 1:500-1:2000
Immunohistochemistry: 1:50-1:200

Datasheet PDF