MAP3K5 Antibody

Catalog Number:

36967

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q99683
NCBI Gene ID: 4217
Gene Accssion: NP_005914

Form of Antibody:

Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

Storage/Stability:

Immunogen:

Synthetic peptide corresponding to residues near the N terminal of human mitogen-activated protein kinase kinase kinase 5

Purification:

Antigen affinity purification.

Specificity/Sensitivity:

The antibody detects endogenous levels of total MAP3K5 protein.

Applications:

IHC

Background:

Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.

References:

appl_detail:

Immunohistochemistry: 1:25-1:100

Datasheet PDF