LAYN Antibody

Catalog Number:

36948

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q6UX15
NCBI Gene ID: 143903
Gene Accssion: NP_001245319

Form of Antibody:

Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

Storage/Stability:

Immunogen:

Synthetic peptide corresponding to residues near the C terminal of human layilin

Purification:

Antigen affinity purification.

Specificity/Sensitivity:

The antibody detects endogenous levels of total LAYN protein.

Applications:

WB

Background:

Layilin is a recently cloned ?55-kDa membrane-binding partner for talin (Borowsky and Hynes, 1998 ) that is widely expressed in different cell types and tissue extracts. It is found in peripheral ruffles of spreading cells and is recruited to membrane ruffles in cells induced to migrate in in vitro wounding experiments. Layilin colocalizes with talin in ruffles and binds to talin's ?50-kDa head domain (amino acids 280¨C435). Other parts of talin (the ?220-kDa tail fragment) can bind β-integrin cytoplasmic tails, vinculin, and F-actin and hence form an integrin-cytoskeleton linkage at sites of cell-substratum contact. Layilin's extracellular domain is homologous with the carbohydrate-recognition domains (CRD) of C-type lectins.

References:

appl_detail:

Western blotting: 1:200-1:1000

Datasheet PDF