DUT Antibody

Catalog Number:

36828

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P33316
NCBI Gene ID: 1854
Gene Accssion: NP_001939

Form of Antibody:

Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

Storage/Stability:

Immunogen:

Synthetic peptide corresponding to residues near the C terminal of human Deoxyuridine triphosphatase

Purification:

Antigen affinity purification.

Specificity/Sensitivity:

The antibody detects endogenous levels of total DUT protein.

Applications:

WB

Background:

This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.

References:

appl_detail:

Western blotting: 1:200-1:1000

Datasheet PDF