APAF1 Antibody

Catalog Number:

36718

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: O14727
NCBI Gene ID: 317
Gene Accssion: NP_863659

Form of Antibody:

Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

Storage/Stability:

Immunogen:

Synthetic peptide corresponding to residues near the C terminal of human Apoptotic protease-activating factor 1

Purification:

Antigen affinity purification.

Specificity/Sensitivity:

The antibody detects endogenous levels of total APAF1 protein.

Applications:

WB

Background:

This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.

References:

appl_detail:

Western blotting: 1:200-1:1000

Datasheet PDF