GCK Antibody

Catalog Number:

36499

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P35557
NCBI Gene ID: 2645
Gene Accssion: BC001890/P35557

Form of Antibody:

Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

Storage/Stability:

Immunogen:

Fusion protein corresponding to a region derived from internal residues of human glucokinase (hexokinase 4)

Purification:

Antigen affinity purification.

Specificity/Sensitivity:

The antibody detects endogenous levels of total GCK protein.

Applications:

WB IHC

Background:

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).

References:

appl_detail:

Western blotting: 1:500-1:2000
Immunohistochemistry: 1:50-1:200

Datasheet PDF